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Image Search Results
Journal: Science Advances
Article Title: Vasculogenic tissue nanotransfection accelerates functional recovery after peripheral nerve injury
doi: 10.1126/sciadv.aeb7631
Figure Lengend Snippet: Immunofluorescence micrographs represent the linage tracing analysis of sciatic nerve tissue 7 days after crush injury followed by EFF or sham TNT, showing GFP linage tracer expression in transgenic mouse models to identify cells originating from ( A ) Schwann cells (S100B) or ( B ) fibroblasts (S100A4). ( C ) Histological analysis revealed increased CD31 expression in the EFF TNT-treated group compared to the sham group in the S100B-EGFP mouse model, demonstrating successful vasculogenic reprogramming. ( D ) However, there was little to no increase in coassociation of the lineage tracer for S100B (GFP, green) with the endothelial marker (CD31, red) and cell nuclei (DAPI, blue) compared to the sham group. ( E ) In contrast, the S100A4-EGFP mouse model showed significantly higher expression of CD31 in the EFF treatment compared to the sham group and ( F ) a significant increase in coassociation between GFP-labeled fibroblasts with CD31 and DAPI in response to EFF TNT, suggesting that fibroblasts are the predominant contributors to the newly formed vascular cell population induced by reprogramming. All error bars are shown as SEM; * denotes significant difference with respect to sham with a P value < 0.05, two-tailed t -test.
Article Snippet: For linage tracing experiments,
Techniques: Immunofluorescence, Expressing, Transgenic Assay, Marker, Labeling, Two Tailed Test